Microinjected Polystyrene Beads Move Along Astral Rays in Sand Dollar Eggs. (astral rays/fertilization/mitosis/microinjected polystyrene beads/sand dollar eggs)
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چکیده
منابع مشابه
Experiments concerning the cleavage stimulus in sand dollar eggs.
It is now clear that the mitotic figure of dividing echinoderm eggs plays a causal role in the determination of the furrow. Since Hiramoto ('56) showed that removal of the mitotic apparatus before appearance of the furrow did not prevent division , it is convenient to consider the apparatus the source of a stimulus which is the immediate trigger for cytokinesis. Neither the area of the cell whi...
متن کاملAn Indirect Method to Assay for Mitotic Centers in Sand Dollar (dendraster Excentricus) Eggs
It is possible consistently to induce sea urchin and sand dollar eggs to cleave directly from one cell into four cells. This is done by exposing the fertilized eggs to benzimidazole for 20 to 30 min beginning about early metaphase. The mitotic apparatus regresses, the cells do not cleave, and shortly after they are returned to normal sea water an early-prophase-appearing nucleus is present in e...
متن کاملThe Origin and Fate of Annulate Lamellae in Maturing Sand Dollar Eggs
Electron micrograph evidence is presented that the nuclear envelope of the mature ovum of Dendraster excentricus is implicated in a proliferation of what appear as nuclear envelope replicas in the cytoplasm. The proliferation is associated with intranuclear vesicles which apparently coalesce to form comparatively simple replicas of the nuclear envelope closely applied to the inside of the nucle...
متن کاملDistribution of fluorescently labeled tubulin injected into sand dollar eggs from fertilization through cleavage
Porcine brain tubulin labeled with fluorescein isothiocyanate (FITC) was able to polymerize by itself and co-polymerize with tubulin purified from starfish sperm flagella. When we injected the FITC-labeled tubulin into unfertilized eggs of the sand dollar, Clypeaster japonicus, and the eggs were then fertilized, the labeled tubulin was incorporated into the sperm aster. When injected into ferti...
متن کاملRedistribution of fluorescently labeled tubulin in the mitotic apparatus of sand dollar eggs and the effects of taxol.
Fluorescently labeled tubulin was quickly incorporated into the mitotic apparatus when injected into a live sand dollar egg. After a rectangular area (1.6 X 16 microns) of the mitotic spindle was photobleached at metaphase or anaphase by the irradiation of a laser microbeam, redistribution of fluorescence was almost complete within 30 sec. The photobleached area did not change in shape during t...
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ژورنال
عنوان ژورنال: Development, Growth and Differentiation
سال: 1986
ISSN: 0012-1592,1440-169X
DOI: 10.1111/j.1440-169x.1986.00461.x